St Johnston Lab | Publications

Xian Yu, Xiao Chen, Mamta Amrute-Nayak, Edward Allgeyer, Aite Zhao, Hannah Chenoweth, Marc Clement, James Harrison, Christian Doreth, George Sirinakis, Thomas Krieg, Huiyu Zhou, Hongda Huang, Kiyotaka Tokuraku, Daniel St Johnston, Ziad Mallat & Xuan Li

Nature (May 2021)

RhoGAP19D inhibits Cdc42 laterally to control epithelial cell shape and prevent invasion

Weronika Fic, Rebecca Bastock, Francesco Raimondi, Erinn Los, Robert B. Russell, Daniel St Johnston

Journal of Cell Biology (March 2021)

2019

The role of integrins in Drosophila egg chamber morphogenesis

Holly E. Lovegrove, Dan T. Bergstralh, Daniel St Johnston

Development (December 2019)

Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags

Roman S Erdmann, Stephanie Wood Baguley, Jennifer H Richens, Rebecca F Wissner, Zhiqun Xi, Edward S Allgeyer, Sheng Zhong, Alexander D Thompson, Nicholas Lowe, Richard Butler, Joerg Bewersdorf, James E Rothman, Daniel St Johnston, Alanna Schepartz, Derek Toomre

Cell Chemical Biology (February 2019)

IMP regulates Kuzbanian to control the timing of Notch signalling in Drosophila follicle cells

Weronika Fic, Celia Faria, Daniel St Johnston

Development (January 2019)

2018

An alternative mode of epithelial polarity in the Drosophila midgut

Jia Chen, Aram-Christopher Sayadian, Nick Lowe, Holly E. Lovegrove, Daniel St Johnston

PLoS Biology (October 2018)

Establishing and transducing cell polarity: common themes and variations

Daniel St Johnston

Current Opinion in Cell Biology (April 2018)

2017

Localised dynactin protects growing microtubules to deliver oska mRNA to the posterior cortex of the Drosophila oocyte

Ross Nieuwburg, Dmitry Nashchekin, Maximilian Jakobs, Andrew P Carter, Philipp Khuc Trong, Raymond E Goldstein, Daniel St Johnston

eLife (October 2017)

aPKC Cycles between functionally distinct PAR protein assemblies to drive cell polarity

Josana Rodriguez, Florent Peglion, Jack Martin, Lars Hubatsch, Jacob Reich, Nisha Hirani, Alicia G Gubieda, Jon Roffey, Artur Ribeiro Fernandes, Daniel St Johnston, Julie Ahringer, Nathan W Goehring

Developmental Cell (August 2017)

Spindle orientation: a question of complex positioning

Dan T. Bergstralh, Nicole S. Dawney, Daniel St Johnston

Development (April 2017)

2016

bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring

Vítor Trovisco, Katsiaryna Belaya, Dmitry Nashchekin, Uwe Irion, George Sirinakis, Richard Butler, Jack J Lee, Elizabeth R Gavis, Daniel St Johnston

eLife (2016)

Patronin/Shot Cortical Foci Assemble the Noncentrosomal Microtubule Array that Specifies the Drosophila Anterior-Posterior Axis

Dmitry Nashchekin, Artur Ribeiro Fernandes, Daniel St Johnston

Developmental Cell (2016)

Pins is not required for spindle orientation in the Drosophila wing disc

Dan T. Bergstralh, Holly E. Lovegrove, Izabela Kujawiak, Nicole S. Dawney, Jinwei Zhu, Samantha Cooper, Rongguang Zhang, Daniel St Johnston

Development (2016)

2015

Lateral adhesion drives reintegration of misplaced cells into epithelial monolayers

Dan T. Bergstralh, Holly E. Lovegrove, Daniel St Johnston

Nature Cell Biology (2015)

Cortical microtubule nucleation can organise the cytoskeleton of Drosophila oocytes to define the anteroposterior axis

Philipp Khuc Trong, Hélène Doerflinger, Jörn Dunkel, Daniel St Johnston, Raymond E Goldstein

eLife (2015)

The renaissance of developmental biology.

Daniel St Johnston

PLoS Biology (2015)

2014

Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library

Nick Lowe, Johanna S Rees, John Roote, Ed Ryder, Irina M Armean, Glynnis Johnson, Emma Drummond, Helen Spriggs, Jenny Drummond, Jose P Magbanua, Huw Naylor, Bénédicte Sanson, Rebecca Bastock, Sven Huelsmann, Vitor Trovisco, Matthias Landgraf, Seymour Knowles-Barley, J Douglas Armstrong, Helen White-Cooper, Celia Hansen, Roger G Phillips, UK Drosophila Protein Trap Screening Consortium; Kathryn S Lilley, Steven Russell, Daniel St Johnston

Development (2014)

Slmb antagonises the aPKC/Par-6 complex to control oocyte and epithelial polarity

Eurico Morais-de-Sá, Avik Mukherjee, Nick Lowe, Daniel St Johnston

Development (2014)

Spindle orientation: What if it goes wrong?

Dan T Bergstralh, Daniel St Johnston

Seminars in Cell & Developmental Biology (2014)

Staufen targets coracle mRNA to Drosophila neuromuscular junctions and regulates GluRIIA synaptic accumulation and bouton number

A Gardiol, Daniel St Johnston

Developmental Biology (2014)

2013

Damage to the Drosophila follicle cell epithelium produces "false clones" with apparent polarity phenotypes

Timm Haack, Dan T Bergstralh, Daniel St Johnston

Biology Open (2013)

Epithelial polarity and spindle orientation: intersecting pathways.

Dan T Bergstralh, Timm Haack, Daniel St Johnston D

Philosophical Transactions of the Royal Society of Biological Sciences (2013)

Using mutants, knockdowns, and transgenesis to investigate gene function in Drosophila

Daniel St Johnston

WIREs Developmental Biology (2013)

Oskar is targeted for degradation by the sequential action of Par-1, GSK-3, and the SCF⁻Slimb ubiquitin ligase

Eurico Morais-de-Sá, Antonio Vega-Rioja A, Vítor Trovisco, Daniel St Johnston

Developmental Cell (2013)

Discs large links spindle orientation to apical-basal polarity in Drosophila epithelia

Dan T Bergstralh, Holly E Lovegrove, Daniel St Johnston

Current Biology (2013)

2012

Epithelial cell polarity: what flies can teach us about cancer

Dan T Bergstralh, Daniel St Johnston

Essays Biochemistry (2012)

Growing microtubules push the oocyte nucleus to polarize the Drosophila dorsal-ventral axis

Tongtong Zhao, Graham OS, Alexandre ASF Raposo, Daniel St Johnston

Science (2012)

2011

Going with the flow: an elegant model for symmetry breaking

Rebecca Bastock, Daniel St Johnston

Developmental Cell (2011)

Epithelial polarity and morphogenesis

Daniel St Johnston, Bénédicte Sanson

Current Opinion in Cell Biology (2011)

Anterior-posterior axis specification in Drosophila oocytes: identification of novel bicoid and oskar mRNA localization factors

Chin-Wen Chang, Dmitry Nashchekin, Lucy Wheatley, Uwe Irion, Katja Dahlgaard, Tessa G Montague, Jacqueline Hall, Daniel St Johnston

Genetics (2011)

In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry

Johanna S Rees, Nick Nick Lowe, Irina M Armean, John Roote, Glynnis Johnson, Emma Drummond, Helen Spriggs, Edward Ryder, Steven Russell, Daniel St Johnston, Kathryn S Lilley

Molecular & Cellular Proteomics (2011)

Using the mRNA-MS2/MS2CP-FP system to study mRNA transport during Drosophila oogenesis

Katsiaryna Belaya, Daniel St Johnston

Methods in Molecular Biology (2011)

Oogenesis: matrix revolutions

Rebecca Bastock, Daniel St Johnston

Current Biology (2011)

2010

Cell polarity in eggs and epithelia: parallels and diversity

Daniel St Johnston, Julie Ahringer

Cell (2010)

aPKC Phosphorylation of Bazooka Defines the Apical/Lateral Border in Drosophila Epithelial Cells

Eurico Morais-de-Sá, Vincent Mirouse, Daniel St Johnston

Cell (2010)

Bazooka is required for polarisation of the Drosophila anterior-posterior axis

Hélène Doerflinger, Nina Vogt, Isabel L Torres, Vincent Mirouse, Iris Koch, Christiane Nüsslein-Volhard, Daniel St Johnston

Development (2010)

2009

Egalitarian recruitment of localized mRNAs

Dmitry Nashchekin, Daniel St Johnston

Genes & Development (2009)

2008

Drosophila oogenesis

Rebecca Bastock, Daniel St Johnston

Current Biology (2008)

Counting motors by force

Daniel St Johnston

Cell (2008)

In Vivo Imaging of oskar mRNA Transport Reveals the Mechanism of Posterior Localization

Vitaly Zimyanin, Katsiaryna Belaya, Jacques Pecreaux, Michael J Gilchrist, Alejandra Clark, Ilan Davis, Daniel St Johnston

Cell (2008)

2007

Assemble a Cytoplasmic Actin Mesh that Maintains Microtubule Organization in the Drosophila Oocyte

Katja Dahlgaard, Alexandre ASF Raposo, Teresa Niccoli, Daniel St Johnston

Capu and Spire

Developmental Cell (2007)

From Stem Cell to Embryo without Centrioles

Naomi R Stevens, Alexandre ASF Raposo, Renata Basto, Daniel St Johnston, Jordan W Raff

Current Biolology (2007)

Drosophila follicle cells are patterned by multiple levels of Notch signaling and antagonism between the Notch and JAK/STAT pathways

Efrat Assa-Kunik, Isabel L Torres, Eyal D Schejter, Daniel St Johnston, Ben-Zion Shilo

Development (2007)

An Oskar-Dependent Positive Feedback Loop Maintains the Polarity of the Drosophila Oocyte

Vitaly Zimyanin, Nick Lowe, Daniel St Johnston

Current Biology (2007)

bicoid RNA localization requires specific binding of an endosomal sorting complex

Uwe Irion, Daniel St Johnston

Nature (2007)

2006

Miranda couples oskar mRNA/Staufen complexes to the bicoid mRNA localization pathway

Uwe Irion, Jan Adams, Chin-Wen Chang, Daniel St Johnston

Developmental Biolology (2006)

Drosophila mus301/spindle-C encodes a helicase with an essential role in double-strand DNA break repair and meiotic progression

Ruth McCaffrey, Daniel St Johnston, Acaimo González-Reyes

Genetics (2006)

Drosophila anterior-posterior polarity requires actin-dependent PAR-1 recruitment to the oocyte posterior

Hélène Doerflinger, Richard Benton, Isabel L Torres, Maarten F Zwart, Daniel St Johnston

Current Biology (2006)

A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMP

Trent Munro, Sunjong Kwon, Bruce J Schnapp, Daniel St Johnston

Journal of Cell Biology (2006)

A novel mutant phenotype implicates dicephalic in cyst formation in the Drosophila ovary

Ruth McCaffrey, Daniel St Johnston, Acaimo González-Reyes

Developmental Dynamics (2006)

2005

Moving messages: the intracellular localization of mRNAs

Daniel St Johnston

Nature Reviews Molecular Cell Biology (2005). Review

2004

The origin of asymmetry: early polarisation of the Drosophila germline cyst and oocyte

Jean-René Huynh, Daniel St Johnston

Current Biology (2004). Review

The Drosophila hnRNPA/B homolog, Hrp48, is specifically required for a distinct step in osk mRNA localization

Jean-René Huynh, Trent Munro, Katie Smith-Litière, Jean-Antoine Lepesant, Daniel St Johnston

Developmental Cell (2004)

An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay

Isabel M Palacios, David Gatfield, Daniel St Johnston, Elisa Izaurralde

Nature (2004)

Seeing is believing: the bicoid morphogen gradient matures

Anne Ephrussi, Daniel St Johnston

Cell (2004). Review

2003

Drosophila PAR-1 and 14-3-3 inhibit Bazooka/PAR-3 to establish complementary cortical domains in polarized cells

Richard Benton, Daniel St Johnston

Cell (2003) Erratum in: Cell (2004)

Notch/Delta-dependent relay mechanism establishes anterior-posterior polarity in Drosophila

Isabel L Torres, Hernán López-Schier, Daniel St Johnston

Developmental Cell (2003)

A conserved oligomerization domain in drosophila Bazooka/PAR-3 is important for apical localization and epithelial polarity

Richard Benton, Daniel St Johnston

Current Biology (2003)

The identification of novel genes required for Drosophila anteroposterior axis formation in a germline clone screen using GFP-Staufen

Sophie G Martin, Vincent Leclerc, Katie Smith-Litière K, Daniel St Johnston

Development (2003)

The role of PAR-1 in regulating the polarised microtubule cytoskeleton in the Drosophila follicular epithelium

Hélène Doerflinger, Richard Benton, Josh M Shulman, Daniel St Johnston

Development (2003)

Barentsz, a new component of the Staufen-containing ribonucleoprotein particles in mammalian cells, interacts with Staufen in an RNA-dependent manner

Paolo Macchi, Sven Kroening, Isabel M Palacios, Simona Baldassa, Barbara Grunewald, Concetta Ambrosino , Bernhard Goetze, Andrei Lupas, Daniel St Johnston, Michael Kiebler

Journal of Neuroscience (2003)

A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity

Sophie G Martin, Daniel St Johnston

Nature (2003)

2002

Drosophila 14-3-3/PAR-5 is an essential mediator of PAR-1 function in axis formation

Richard Benton, Isabel M Palacios, Daniel St Johnston

Developmental Cell (2002)

Kinesin light chain-independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte

Isabel M Palacios, Daniel St Johnston

Development (2002)

Cell polarity: posterior Par-1 prevents proteolysis

Richard Benton, Daniel St Johnston

Current Biology (2002). Review

The art and design of genetic screens: Drosophila melanogaster

Daniel St Johnston

Nature Reviews Genetics (2002). Review

Drosophila nicastrin is essential for the intramembranous cleavage of notch

Hernán López-Schier, Daniel St Johnston

Developmental Cell (2002)

2001

The beginning of the end

Daniel St Johnston

EMBO Journal (2001)

Getting the message across: the intracellular localization of mRNAs in higher eukaryotes

Isabel M Palacios, Daniel St Johnston

Annual Review of Cell and Developmental Biology (2001). Review

A rapid method to map mutations in Drosophila

Sophie G Martin, Krista C Dobi, Daniel St Johnston

Genome Biology (2001)

Bazooka and PAR-6 are required with PAR-1 for the maintenance of oocyte fate in Drosophila

Jean-René Huynh, Mark Petronczki, Jürgen A Knoblich, Daniel St Johnston

Current Biology (2001)

Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole

FJ van Eeden, Isabel M Palacios, Mark Petronczki, MJ Weston, Daniel St Johnston

Journal of Cell Biolology (2001)

Delta signaling from the germ line controls the proliferation and differentiation of the somatic follicle cells during Drosophila oogenesis

Hernán López-Schier, Daniel St Johnston

Genes & Development (2001)

Centrosome migration into the Drosophila oocyte is independent of BicD and egl, and of the organisation of the microtubule cytoskeleton

Bolívar J, Jean-René Huynh, Hernán López-Schier, González C, St Johnston D, Acaimo González-Reyes

Development. (2001)

PAR-1 is required for the maintenance of oocyte fate in Drosophila

Jean-René Huynh, Josh M Shulman, Richard Benton, Daniel St Johnston

Development (2001)

2000

The role of BicD, Egl, Orb and the microtubules in the restriction of meiosis to the Drosophila oocyte

Jean-René Huynh, Daniel St Johnston

Development (2000)

The Drosophila homolog of C. elegans PAR-1 organizes the oocyte cytoskeleton and directs oskar mRNA localization to the posterior pole

Josh M Shulman, Richard Benton, Daniel St Johnston

Cell (2000)

Distinct roles of two conserved Staufen domains in oskar mRNA localization and translation

David R Micklem, Jan Adams, Stefan Grünert, Daniel St Johnston

EMBO Journal (2000)

1999

Pattern formation in single cells

Josh M Shulman, Daniel St Johnston

Trends Cell Biology (1999). Review

The polarisation of the anterior-posterior and dorsal-ventral axes during Drosophila oogenesis

Fredericus van Eeden, Daniel St Johnston

Current Opinion in Genetics & Development (1999). Review

1998

The Drosophila AP axis is polarised by the cadherin-mediated positioning of the oocyte

Acaimo González-Reyes, Daniel St Johnston

Development (1998)

Patterning of the follicle cell epithelium along the anterior-posterior axis during Drosophila oogenesis

Acaimo González-Reyes, Daniel St Johnston

Development (1998)

1997

Oocyte determination and the origin of polarity in Drosophila: the role of the spindle genes

Acaimo González-Reyes, Elliott H, Daniel St Johnston

Development (1997)

The mago nashi gene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila

David R Micklem, R Dasgupta, H Elliott, Fanni Gergely, Catherine Davidson C, Andrea Brand, Acaimo González-Reyes, Daniel St Johnston

Current Biology (1997)

1996

RNA localization and the development of asymmetry during Drosophila oogenesis

Stefan Grünert, Daniel St Johnston

Current Opinion in Genetics & Development (1996)

1995

New role for tropomyosin

Daniel St Johnston

Developmental biology. Nature (1995)

Polarization of both major body axes in Drosophila by gurken-torpedo signalling

Acaimo González-Reyes, Elliott H, Daniel St Johnston

Nature (1995)

The intracellular localization of messenger RNAs

Daniel St Johnston

Cell (1995)

1994

Role of oocyte position in establishment of anterior-posterior polarity in Drosophila

Acaimo González-Reyes, Daniel St Johnston

Science (1994)

RNA localization. Getting to the top

Daniel St Johnston

Current Biology (1994)

1992

A conserved double-stranded RNA-binding domain

Daniel St Johnston, Nick H Brown, JG Gall, M Jantsch

PNAS (1992)