St Johnston Lab

Publications from the lab:

Holly E. Lovegrove, Dan T. Bergstralh, Daniel St Johnston. The role of integrins in Drosophila egg chamber morphogenesis. bioRxiv; doi: 10.1101/706069.

Weronika Fic, Celia Faria, Daniel St Johnston. IMP regulates Kuzbanian to control the timing of Notch signalling in Drosophila follicle cells. Development. 2019 146; doi: 10.1242/dev.168963

Jia Chen, Aram-Christopher Sayadian, Nick Lowe, Holly E. Lovegrove, Daniel St Johnston. An alternative mode of epithelial polarity in the Drosophila midgut. PLoS Biol. 2018 Oct 19;16(10):e3000041; doi: 110.1371/journal.pbio.3000041

Ross Nieuwburg, Dmitry Nashchekin, Maximilian Jakobs, Andrew P Carter, Philipp Khuc Trong, Raymond E Goldstein, Daniel St Johnston. Localised dynactin protects growing microtubules to deliver oska mRNA to the posterior cortex of the Drosophila oocyte. eLife 2017;6:e27237 doi: 10.7554/eLife.27237

Dan T. Bergstralh, Nicole S. Dawney, Daniel St Johnston. Spindle orientation: a question of complex positioning. Development 2017 144: 1137-1145; doi: 10.1242/dev.140764

Vítor Trovisco, Katsiaryna Belaya, Dmitry Nashchekin, Uwe Irion, George Sirinakis, Richard Butler, Jack J Lee, Elizabeth R Gavis, Daniel St Johnston. bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring. eLife. 2016;5:e17537

Dmitry Nashchekin, Artur Ribeiro Fernandes, Daniel St Johnston. Patronin/Shot Cortical Foci Assemble the Noncentrosomal Microtubule Array that Specifies the Drosophila Anterior-Posterior Axis. Developmental Cell. 2016 Jun 11. Volume 38, Issue 1, p61–72

Dan T. Bergstralh, Holly E. Lovegrove, Izabela Kujawiak, Nicole S. Dawney, Jinwei Zhu, Samantha Cooper, Rongguang Zhang, Daniel St Johnston. Pins is not required for spindle orientation in the Drosophila wing disc. Development. 2016 Jun 10. 143: 2573-2581; doi: 10.1242/dev.135475

Dan T. Bergstralh, Holly E. Lovegrove & Daniel St Johnston. Lateral adhesion drives reintegration of misplaced cells into epithelial monolayers. Nature Cell Biology (2015) Nov;17(11):1497-503 doi:10.1038/ncb3248. Epub 2015 Sep 28.

Philipp Khuc Trong, Hélène Doerflinger, Jörn Dunkel, Daniel St Johnston, Raymond E Goldstein. Cortical microtubule nucleation can organise the cytoskeleton of Drosophila oocytes to define the anteroposterior axis. Elife. 2015 Sep 25;4. doi: 10.7554/eLife.06088.

St Johnston D. The renaissance of developmental biology.PLoS Biol. 2015 May 6;13(5):e1002149.

Lowe N, Rees JS, Roote J, Ryder E, Armean IM, Johnson G, Drummond E, Spriggs H, Drummond J, Magbanua JP, Naylor H, Sanson B, Bastock R, Huelsmann S, Trovisco V, Landgraf M, Knowles-Barley S, Armstrong JD, White-Cooper H, Hansen C, Phillips RG; UK Drosophila Protein Trap Screening Consortium, Lilley KS, Russell S, St Johnston D. Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library. Development. 2014 Oct;141(20):3994-4005.

Morais-de-Sá E, Mukherjee A, Lowe N, St Johnston D. Slmb antagonises the aPKC/Par-6 complex to control oocyte and epithelial polarity. Development. 2014 Aug;141(15):2984-92.

Bergstralh DT, St Johnston D. Spindle orientation: What if it goes wrong? Semin Cell Dev Biol. 2014 Jun 24.

Gardiol A, St Johnston D. Staufen targets coracle mRNA to Drosophila neuromuscular junctions and regulates GluRIIA synaptic accumulation and bouton number. Dev Biol. 2014 Aug 15;392(2):153-67.

Haack T, Bergstralh DT, St Johnston D. Damage to the Drosophila follicle cell epithelium produces "false clones" with apparent polarity phenotypes. Biol Open. 2013 Dec 15;2(12):1313-20.

Bergstralh DT, Haack T, St Johnston D. Epithelial polarity and spindle orientation: intersecting pathways. Philos Trans R Soc Lond B Biol Sci. 2013 Sep 23;368(1629):20130291.

St Johnston D. Using mutants, knockdowns, and transgenesis to investigate gene function in Drosophila. Wiley Interdiscip Rev Dev Biol. 2013 Sep-Oct;2(5):587-613.

Morais-de-Sá E, Vega-Rioja A, Trovisco V, St Johnston D. Oskar is targeted for degradation by the sequential action of Par-1, GSK-3, and the SCF⁻Slimb ubiquitin ligase. Dev Cell. 2013 Aug 12;26(3):303-14.

Bergstralh DT, Lovegrove HE, St Johnston D. Discs large links spindle orientation to apical-basal polarity in Drosophila epithelia. Curr Biol. 2013 Sep 9;23(17):1707-12.

Bergstralh DT, St Johnston D. Epithelial cell polarity: what flies can teach us about cancer. Essays Biochem. 2012;53:129-40.

Zhao T, Graham OS, Raposo A, St Johnston D. Growing microtubules push the oocyte nucleus to polarize the Drosophila dorsal-ventral axis. Science. 2012 May 25;336(6084):999-1003.

Bastock R, St Johnston D. Going with the flow: an elegant model for symmetry breaking. Dev Cell. 2011 Dec 13;21(6):981-2.

St Johnston D, Sanson B. Epithelial polarity and morphogenesis. Curr Opin Cell Biol. 2011 Oct;23(5):540-6.

Chang CW, Nashchekin D, Wheatley L, Irion U, Dahlgaard K, Montague TG, Hall J, St Johnston D. Anterior-posterior axis specification in Drosophila oocytes: identification of novel bicoid and oskar mRNA localization factors. Genetics. 2011 Aug;188(4):883-96.

Rees JS, Lowe N, Armean IM, Roote J, Johnson G, Drummond E, Spriggs H, Ryder E, Russell S, St Johnston D, Lilley KS. In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry. Mol Cell Proteomics. 2011 Jun;10(6):M110.002386.

Belaya K, St Johnston D. Using the mRNA-MS2/MS2CP-FP system to study mRNA transport during Drosophila oogenesis. Methods Mol Biol. 2011;714:265-83.

Bastock R, St Johnston D. Oogenesis: matrix revolutions. Curr Biol. 2011 Mar 22;21(6):R231-3.

St Johnston D, Ahringer J. Cell polarity in eggs and epithelia: parallels and diversity. Cell. 2010 May 28;141(5):757-74.

Morais-de-Sá E, Mirouse V, St Johnston D. aPKC Phosphorylation of Bazooka Defines the Apical/Lateral Border in Drosophila Epithelial Cells. Cell. 2010 Apr 30;141(3):509-23.

Doerflinger H, Vogt N, Torres IL, Mirouse V, Koch I, Nüsslein-Volhard C, St Johnston D. Bazooka is required for polarisation of the Drosophila anterior-posterior axis. Development. 2010 May;137(10):1765-73.

Nashchekin D, St Johnston D. Egalitarian recruitment of localized mRNAs. Genes Dev. 2009 Jul 1;23(13):1475-80. (Comment on: Genes Dev. 2009 Jul 1;23(13):1546-58.)

Bastock R, St Johnston D. Drosophila oogenesis. Curr Biol. 2008 Dec 9; 18(23): R1082-7.

St Johnston D. Counting motors by force. Cell. 2008 Dec 12;135(6):1000-1.

Zimyanin VL, Belaya K, Pecreaux J, Gilchrist MJ, Clark A, Davis I, St Johnston D. In Vivo Imaging of oskar mRNA Transport Reveals the Mechanism of Posterior Localization. Cell. 2008 Sep 5;134(5):843-853.

Dahlgaard K, Raposo AA, Niccoli T, St Johnston D. Capu and Spire Assemble a Cytoplasmic Actin Mesh that Maintains Microtubule Organization in the Drosophila Oocyte. Dev Cell. 2007 Oct 9;13(4):539-53.

Stevens NR, Raposo AA, Basto R, St Johnston D, Raff JW. From Stem Cell to Embryo without Centrioles. Curr Biol. 2007 Sep 4;17(17):1498-503.

Assa-Kunik E, Torres IL, Schejter ED, St Johnston D, Shilo BZ. Drosophila follicle cells are patterned by multiple levels of Notch signaling and antagonism between the Notch and JAK/STAT pathways. Development. 2007 Mar 1;134(6):1161-9.

Zimyanin V, Lowe N, St Johnston D. An Oskar-Dependent Positive Feedback Loop Maintains the Polarity of the Drosophila Oocyte. Current Biology. 2007 Feb 20;17(4):353-9.

Irion U, St Johnston D. bicoid RNA localization requires specific binding of an endosomal sorting complex. Nature. 2007 Feb 1;445(7127):554-8.

Irion U, Adams J, Chang CW, St Johnston D. Miranda couples oskar mRNA/Staufen complexes to the bicoid mRNA localization pathway. Dev Biol. 2006 Sep 15;297(2):522-33.

McCaffrey R, St Johnston D, Gonzalez-Reyes A. Drosophila mus301/spindle-C encodes a helicase with an essential role in double-strand DNA break repair and meiotic progression. Genetics. 2006 Nov;174(3):1273-85.

Doerflinger H, Benton R, Torres IL, Zwart MF, St Johnston D. Drosophila anterior-posterior polarity requires actin-dependent PAR-1 recruitment to the oocyte posterior. Curr. Biol. 2006 Jun 6;16(11): 1090-5.

Munro TP, Kwon S, Schnapp BJ, St Johnston D. A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMP. J Cell Biol. 2006 Feb 13;172(4):577-88.

McCaffrey R, St Johnston D, González-Reyes A. A novel mutant phenotype implicates dicephalic in cyst formation in the Drosophila ovary. Dev Dyn. 2006 Apr;235(4):908-17.

St Johnston D. Moving messages: the intracellular localization of mRNAs. Nat Rev Mol Cell Biol. 2005 May;6(5):363-75. Review.

Huynh JR, St Johnston D. The origin of asymmetry: early polarisation of the Drosophila germline cyst and oocyte. Curr Biol. 2004 Jun 8;14(11):R438-49. Review.

Huynh JR, Munro TP, Smith-Litière K, Lepesant JA, St Johnston D. The Drosophila hnRNPA/B homolog, Hrp48, is specifically required for a distinct step in osk mRNA localization. Dev Cell. 2004 May;6(5):625-35.

Palacios IM, Gatfield D, St Johnston D, Izaurralde E. An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay. Nature. 2004 Feb 19;427(6976):753-7.

Ephrussi A, St Johnston D. Seeing is believing: the bicoid morphogen gradient matures. Cell. 2004 Jan 23;116(2):143-52. Review.

Benton R, St Johnston D. Drosophila PAR-1 and 14-3-3 inhibit Bazooka/PAR-3 to establish complementary cortical domains in polarized cells. Cell. 2003 Dec 12;115(6):691-704. Erratum in: Cell. 2004 Jan 9;116(1):139.

Torres IL, López-Schier H, St Johnston D. A Notch/Delta-dependent relay mechanism establishes anterior-posterior polarity in Drosophila. Dev Cell. 2003 Oct;5(4):547-58.

Benton R, St Johnston D. A conserved oligomerization domain in drosophila Bazooka/PAR-3 is important for apical localization and epithelial polarity. Curr Biol. 2003 Aug 5;13(15):1330-4.

Martin SG, Leclerc V, Smith-Litière K, St Johnston D. The identification of novel genes required for Drosophila anteroposterior axis formation in a germline clone screen using GFP-Staufen. Development. 2003 Sep;130(17):4201-15.

Doerflinger H, Benton R, Shulman JM, St Johnston D. The role of PAR-1 in regulating the polarised microtubule cytoskeleton in the Drosophila follicular epithelium. Development. 2003 Sep;130(17):3965-75.

Macchi P, Kroening S, Palacios IM, Baldassa S, Grunewald B, Ambrosino C, Goetze B, Lupas A, St Johnston D, Kiebler M. Barentsz, a new component of the Staufen-containing ribonucleoprotein particles in mammalian cells, interacts with Staufen in an RNA-dependent manner. J Neurosci. 2003 Jul 2;23(13):5778-88.

Martin SG, St Johnston D. A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity. Nature. 2003 Jan 23;421(6921):379-84.

Benton R, Palacios IM, St Johnston D. Drosophila 14-3-3/PAR-5 is an essential mediator of PAR-1 function in axis formation. Dev Cell. 2002 Nov;3(5):659-71.

Palacios IM, St Johnston D. Kinesin light chain-independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte. Development. 2002 Dec;129(23):5473-85.

Benton R, St Johnston D. Cell polarity: posterior Par-1 prevents proteolysis. Curr Biol. 2002 Jul 23;12(14):R479-81. Review.

St Johnston D. The art and design of genetic screens: Drosophila melanogaster. Nat Rev Genet. 2002 Mar;3(3):176-88. Review.

López-Schier H, St Johnston D. Drosophila nicastrin is essential for the intramembranous cleavage of notch. Dev Cell. 2002 Jan;2(1):79-89.

St Johnston D. The beginning of the end. EMBO J. 2001 Nov 15;20(22):6169-79. Review.

Palacios IM, St Johnston D. Getting the message across: the intracellular localization of mRNAs in higher eukaryotes. Annu Rev Cell Dev Biol. 2001;17:569-614. Review.

Martin SG, Dobi KC, St Johnston D. A rapid method to map mutations in Drosophila. Genome Biol. 2001;2(9):RESEARCH0036.

Huynh JR, Petronczki M, Knoblich JA, St Johnston D. Bazooka and PAR-6 are required with PAR-1 for the maintenance of oocyte fate in Drosophila. Curr Biol. 2001 Jun 5;11(11):901-6.

van Eeden FJ, Palacios IM, Petronczki M, Weston MJ, St Johnston D. Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole. J Cell Biol. 2001 Aug 6;154(3):511-23.

López-Schier H, St Johnston D. Delta signaling from the germ line controls the proliferation and differentiation of the somatic follicle cells during Drosophila oogenesis. Genes Dev. 2001 Jun 1;15(11):1393-405.

Bolívar J, Huynh JR, López-Schier H, González C, St Johnston D, González-Reyes A. Centrosome migration into the Drosophila oocyte is independent of BicD and egl, and of the organisation of the microtubule cytoskeleton. Development. 2001 May;128(10):1889-97.

Huynh JR, Shulman JM, Benton R, St Johnston D. PAR-1 is required for the maintenance of oocyte fate in Drosophila. Development. 2001 Apr;128(7):1201-9.

Huynh JR, St Johnston D. The role of BicD, Egl, Orb and the microtubules in the restriction of meiosis to the Drosophila oocyte. Development. 2000 Jul;127(13):2785-94.

Shulman JM, Benton R, St Johnston D. The Drosophila homolog of C. elegans PAR-1 organizes the oocyte cytoskeleton and directs oskar mRNA localization to the posterior pole. Cell. 2000 May 12;101(4):377-88.

Micklem DR, Adams J, Grünert S, St Johnston D. Distinct roles of two conserved Staufen domains in oskar mRNA localization and translation. EMBO J. 2000 Mar 15;19(6):1366-77.

Shulman JM, St Johnston D. Pattern formation in single cells. Trends Cell Biol. 1999 Dec;9(12):M60-4. Review.

van Eeden F, St Johnston D. The polarisation of the anterior-posterior and dorsal-ventral axes during Drosophila oogenesis. Curr Opin Genet Dev. 1999 Aug;9(4):396-404. Review.

González-Reyes A, St Johnston D. The Drosophila AP axis is polarised by the cadherin-mediated positioning of the oocyte. Development. 1998 Sep;125(18):3635-44.

González-Reyes A, St Johnston D. Patterning of the follicle cell epithelium along the anterior-posterior axis during Drosophila oogenesis. Development. 1998 Aug;125(15):2837-46.

González-Reyes A, Elliott H, St Johnston D. Oocyte determination and the origin of polarity in Drosophila: the role of the spindle genes. Development. 1997 Dec;124(24):4927-37.

Micklem DR, Dasgupta R, Elliott H, Gergely F, Davidson C, Brand A, González-Reyes A, St Johnston D. The mago nashi gene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila. Curr Biol. 1997 Jul 1;7(7):468-78.

Grünert S, St Johnston D. RNA localization and the development of asymmetry during Drosophila oogenesis. Curr Opin Genet Dev. 1996 Aug;6(4):395-402. Review.

St Johnston D. Developmental biology. New role for tropomyosin. Nature. 1995 Oct 12;377(6549):483.

González-Reyes A, Elliott H, St Johnston D. Polarization of both major body axes in Drosophila by gurken-torpedo signalling. Nature. 1995 Jun 22;375(6533):654-8.

St Johnston D. The intracellular localization of messenger RNAs. Cell. 1995 Apr 21;81(2):161-70.

González-Reyes A, St Johnston D. Role of oocyte position in establishment of anterior-posterior polarity in Drosophila. Science. 1994 Oct 28;266(5185):639-42.

St Johnston D. RNA localization. Getting to the top. Curr Biol. 1994 Jan 1;4(1):54-6.

St Johnston D, Brown NH, Gall JG, Jantsch M. A conserved double-stranded RNA-binding domain. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10979-83.

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