Selected publications
Papers:
Izawa, D. and Pines, J. (2014) ‘The Mitotic Checkpoint Complex binds a second CDC20 to inhibit active APC/C’ Nature 517, 631-634
Grallert, A., Boke, E., Hagting, A., Hodgson, B., Connolly,Y., Griffiths, J., Smith, D. Pines, J. and Hagan, I.M. ‘A PP1/PP2A phosphatase relay controls mitotic progression.’ Nature 517, 94-98
Di Fiore, B., Davey, N., Hagting, A., Izawa, D., Gibson, T. and Pines, J. (2015) ‘The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators’ Dev Cell 32, 358-372.
Collin, P., Nashchekina, O., Walker, R. and Pines, J. (2013) ‘The Spindle Assembly Checkpoint works like a rheostat not a toggle-switch’ Nat. Cell Biol.11, 1378 -1385.
Izawa, D. and Pines, J. (2012) 'Mad2 and the APC/C compete for the same site on Cdc20 to ensure proper chromosome segregation'
J. Cell Biol. 199, 27-37.
Mansfeld, J., Collin, P., Collins, M.O., Choudhary, J. and Pines, J. (2011) ‘APC15 drives the turnover of MCC-Cdc20 to make the Spindle Assembly Checkpoint responsive to kinetochore attachment’
Nat. Cell Biol. 13, 1234-1243.
Pagliuca, F., Collins, M.O., Lichawska, A., Zegerman, P., Choudhary, J.S. and Pines, J. (2011) ‘Quantitative Proteomics Reveals the Basis for the Biochemical Specificity of the Cell Cycle Machinery’
Mol Cell 43, 406-417.
Izawa, D. and Pines, J. (2011) 'Evidence for how APC/C-Cdc20 changes its substrate specificity in mitosis'
Nat. Cell Biol.13, 223-233.
Di Fiore, B. and Pines, J. (2010) 'How Cyclin A destruction escapes the Spindle Assembly Checkpoint'
J. Cell Biol.190, 501-509.
Gavet, O. and Pines, J. (2010) 'Progressive activation of Cyclin B1-Cdk1 coordinates entry to mitosis'
Dev. Cell 18, 533-543.
Gavet, O. and Pines, J. (2010) 'Activation of Cyclin B1-Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosis'
J. Cell Biol. 189, 247-259.
Garnett, M.J., Mansfeld, J., Godwin, C., Matsusaka, T., Wu, J., Russell, P., Pines, J., Venkitaraman, A. (2009) 'UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit'
Nat. Cell Biol.11, 1363-1369.
Elder, A.D., Domin, A., Kaminski Schierle, G.S., Lindon, C., Pines, J., Esposito, A. and Kaminski, C.F. (2009) 'A quantitative protocol for dynamic measurements of protein interactions by Förster resonance energy transfer-sensitized fluorescence emission'
J. R. Soc Interface 6, 59-81.
Nilsson, J., Yekezare, M., Minshull , J. and Pines, J. (2008) 'The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction.'
Nat. Cell Biol.10, 1411-1420.
Floyd, S., Pines, J., and Lindon, C. (2008). 'APC/CCdh1 targets Aurora kinase to control reorganization of the mitotic spindle at anaphase.'
Curr Biol. 18, 1649-1658.
Wolthuis, R., Clay-Farrace, L., van Zon, W., Yekezare, M., Ogink, J., Medema, R. and Pines, J. (2008) 'Cdc20 and Cks Direct the Spindle Checkpoint-Independent Destruction of Cyclin A.'
Mol. Cell 30, 290-302.
Walker, A., Acquaviva, C., Matsusaka, M., Koop, L. and Pines, J. (2008) 'UbcH10 has a rate-limiting role in G1 phase but may not act in the spindle checkpoint or as part of an autonomous oscillator.'
J. Cell Sci.121 2319-2326.
Ahel, I., Ahel, D., Matsukaka, T., Clark, A.J., Pines, J., Boulton, S.J., and West, S.C. (2008). 'PBZ: a Novel Poly(ADP-ribose)-Binding Zinc Finger Motif in DNA Repair/Checkpoint Proteins.'
Nature 451, 81-85.
Di Fiore, B and Pines, J. (2007) 'Emi1 is required to couple replication with mitosis but does not regulate activation of the mitotic APC/C.'
J. Cell Biol. 177, 425-437.
Acquaviva, C., Herzog, F., Kraft, K. and Pines, J. (2004). 'The Anaphase Promoting Complex/Cyclosome is recruited to centromeres by the spindle assembly checkpoint.'
Nat. Cell Biol. 6, 892-898
Matsusaka, T., and Pines, J. (2004). 'Chfr acts with the p38 stress kinase to block entry to mitosis in mammalian cells.'
J. Cell Biol. 166, 507-516.
Lindon, C. and Pines, J.,(2004) 'Ordered proteolysis in anaphase inactivates Plk1 to contribute to proper mitotic exit in human cells'
J. Cell Biol. 164, 233-241.
Jackman, M., Lindon, C., Nigg, E.A. and Pines, J. (2003). 'Active cyclin B1-Cdk1 first appears on centrosomes at the end of prophase.'
Nat. Cell Biol. 5, 143-148.
Kraft, C., Herzog, F., Gieffers, C., Mechtler, K., Hagting, A., Pines, J., and Peters, J-M. (2003).
'Mitotic regulation of the human anaphase promoting complex by phosphorylation. '
EMBO Journal 22, 6598-6609.
Hagting, A., den Elzen, N. ,Vodermaier, H.C., Waizenegger, I.C., Peters, J-M. and Pines, J. (2002). 'Human securin proteolysis is controlled by the spindle checkpoint and reveals when the APC/C switches from activation by Cdc20 to Cdh1.'
J. Cell Biol. 157, 1125-1137.
den Elzen, N. and Pines, J. (2001). 'Cyclin A is destroyed in prometaphase and can delay chromosome alignment and anaphase.'
J. Cell Biol. 153, 121-135.
Draviam, V.M., Orrechia, S., Lowe, M., Pardi, R. and Pines, J. (2001) 'The localisation of human cyclins B1 and B2 determines CDK1 substrate specificity and neither enzyme requires MEK to disassemble the Golgi apparatus.'
J. Cell Biol. 152, 945-958.
Clute, P and Pines, J. (1999) 'Temporal and spatial control of Cyclin B1 destruction in metaphase.'
Nat. Cell Biology 1, 82-85.
Hagting, A., Jackman, M., Simpson, K. and Pines, J. (1999) 'The translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal.'
Current Biol. 9, 680-689.
Furuno, N., den Elzen, N. and Pines, J. (1999) 'Human cyclin A is required for mitosis until late prophase.'
J. Cell Biol. 147, 295-306.
Hagting, A., Karlsson, C., Clute, P., Jackman, M. and Pines, J. (1998) 'MPF localisation is controlled by nuclear export.'
EMBO Journal., 17, 4127-4138.
Reviews:
Pines, J. and Hagan, I. (2011) ‘The Renaissance or the Cukoo clock’. Philosophical transactions of the Royal Society of London. Series B, Biological sciences (Special issue in honour of Paul Nurse) in press.
Pines, J. (2011) ‘Cubism and the cell cycle: the multi-faceted APC/C’.
Nature Reviews Molecular Cell Biology 12, 427-438.
Khodjakov, A. and Pines, J. (2010). ‘Centromere tension: a divisive issue’.
Nat. Cell Biol. 12, 919-923.
Pines, J. (2005) 'Mitosis: a matter of getting rid of the right protein at the right time.'
Trends Cell Biol. 16, 55-63.
Pines, J. and Lindon, C (2005) 'Proteolysis: anytime, any place, anywhere?'
Nat. Cell Biol. 7, 731-735
Pines, J. and Rieder, C. L. (2001) 'Re-staging mitosis: a contemporary view of mitotic progression'
Nat. Cell Biol. 3, E3-E6.
Pines, J. (1999) 'Four-Dimensional control of the cell cycle.'
Nat. Cell Biol. 1, E73-E79.
Pablo Picasso: The Guitar Player (1910)