The organisation of the genome within the nucleus is an important aspect of genome regulation that is difficult to address through chromatin profiling experiments. Recent advances in fluorescence microscopy have broken the diffraction barrier, fundamentally changing our ability to visualize structures within cells, with super-resolution microscopic approaches having resolving power down to ~20nm. Because C. elegans develops through an invariant and known cell lineage, it provides a unique system to study the progression of genome organisation and factor that control organization in individual nuclei of known fate. We use dSTORM microscopy to study how genome architecture is regulated in development.
